Size：

0.5ml、100ul，(2mg/ml)

Target：

Human

Host：

Goat

Antibody Format：

Whole IgG

Type

Secondary Antibody

Specificity

human IgG (H+L))

Conjugate：

KELAF™ 488

Product Category：

Whole IgG Affinity-Purified Antibodies

Clonality：

Polyclonal

Information：

To minimize cross-reactivity, these goat anti-human   IgG (H+L) whole secondary antibodies have been affinity purified and   cross-adsorbed against mouse, rabbit, and bovine serum prior to conjugation.   Cross-adsorption or pre-adsorption is a purification step to increase   specificity of the antibody resulting in higher sensitivity and less   background staining. The secondary antibody solution is passed through a   column matrix containing immobilized serum proteins from potentially   cross-reactive species. Only the nonspecific-binding secondary antibodies are   captured in the column, and the highly specific secondaries flow through. The   benefits of this extra step are apparent in multiplexing/multicolor-staining   experiments (e.g., flow cytometry) where there is potential cross-reactivity   with other primary antibodies or in tissue/cell fluorescent staining   experiments where there are may be the presence of endogenous   immunoglobulins.

 KELAF dyes   are among the most trusted fluorescent dyes available today.KELAF™ 488 dye is   a bright, green-fluorescent dye with excitation ideally suited to the 488 nm   laser line. For stable signal generation in imaging and flow cytometry,   KELAF™ 488 dye is pH-insensitive over a wide molar range. Probes with high   fluorescence quantum yield and high photostability allow detection of   low-abundance biological structures with great sensitivity. KELAF™ 488 dye   molecules can be attached to proteins at high molar ratios without   significant self-quenching, enabling brighter conjugates and more sensitive   detection. The degree of labeling for each conjugate is typically 2-8   fluorophore molecules per IgG molecule; the exact degree of labeling is   indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein   conjugate solution briefly in a microcentrifuge before use; add only the   supernatant to the experiment. This step will help eliminate any protein   aggregates that may have formed during storage, thereby reducing nonspecific   background staining. Because staining protocols vary with application, the   appropriate dilution of antibody should be determined empirically. For the   fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be   satisfactory for most immunohistochemistry and flow cytometrya pplications.

Product will be shipped at Room Temperature.

Usage：

Flow Cytometry (Flow) ：1-10   µg/mL

KELAF™ 488：

Amax: 493 Emax: 519nm

KELAF™ 488-conjugated antibodies absorb light   maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous   mounting media they are brighter than FITC, Cy2, and DyLight 488. KELAF™ 488   conjugates are recommended for maximum sensitivity for all immunofluorescence   procedures requiring a green-fluorescing dye, except for protocols that   include mounting in plastic mounting media