产品介绍
FITC anti-human CD8a Recombinant Antibody
Catalog# / Size：A-0014-41 / 25 tests
A-0014-42 / 100 tests
Clone：HIT8a
Regulatory Status：RUO
Workshop：V CD08.10
Other Names：T8, Leu2
Isotype：human IgG1, κ
Description：CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusivelybCD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Product Details
Verified Reactivity：Human
Reported Reactivity：Chimpanzee, Common Marmoset
Antibody Type：Antibody Type Recombinan
Host Species：Mouse
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation：The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions
Concentration
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application：FC-Quality tested
Excitation Laser：Blue Laser (488 nm)
Application Notes：Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.Additional reported applications of this antibody (for the relevant formats) include:immunohistochemical staining of bingtong-fixed frozen tissue sections5,6. This clone was tested in- house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
Antigen Details
Structure：Ig superfamily, homodimer or heterodimer with CD8β , 32-34 kD
Distribution：Majority of thymocytes, T cell subset, NK cells
Function：MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor：MHC Class I molecules
Cell Type：Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area：Immunology
Molecular Family：CD Molecules
Antigen References：1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID：925
Data：
Human peripheral blood lymphocytes are stained with FITC Anti-Human CD8a Antibody.
Unstained lymphocytes (empty black histogram) are used as control.
