Size：

0.5ml，2mg/ml

Target：

Human

Host：

Goat

Antibody Format：

Whole IgG

Specificity

IgG (H+L)

Conjugate：

KELAF™ 647

Product Category：

Whole IgG Affinity-Purified Antibodies

Clonality：

Polyclonal

RRID：

AB_2337880

Information：

Based on immunoelectrophoresis and/or ELISA, the   antibody reacts with whole molecule human IgG. It also reacts with the light   chains of other human immunoglobulins. No antibody was detected against   non-immunoglobulin serum proteins. The antibody may cross-react with   immunoglobulins from other species.

Whole IgG antibodies are isolated as intact   molecules from antisera by immunoaffinity chromatography. They have an Fc   portion and two antigen binding Fab portions joined together by disulfide   bonds and therefore they are divalent. The average molecular weight is   reported to be about 160 kDa. The whole IgG form of antibodies is suitable   for the majority of immunodetection procedures and is the most cost   effective.

Usage：

Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for most applications

KELAF™ 647：

KELAF ™ 647-conjugated antibodies absorb light   maximally around 651 nm and fluoresce maximally around 667 nm. They are   brighter than Cy5 and DyLight 650 in aqueous mounting media. KELAF ™ 647- and APC-conjugated secondary antibodies are the best choice   for flow cytometry when secondary antibodies fluorescing at these   wavelengths are desired. KELAF™ 647 conjugates   are the best choice of far red-emitting dyes for multiple-labeling detection   with a confocal microscope.

A significant advantage of using KELAF ™   647 over lower wavelength-emitting dyes is the low autofluorescence of   biological specimens in this region of the spectrum. However, because of   its peak emission at 667 nm, KELAF ™ 647 cannot   be seen well by eye, and it cannot be excited optimally with a mercury   lamp. Therefore, KELAF ™ 647 is not recommended for   use with conventional epifluorescent microscopes. It is most commonly   visualized with a confocal microscope equipped with an appropriate laser   for excitation and a far-red detector. KELAF™   647 conjugates are less expensive alternatives to allophycocyanin   conjugates for flow cytometry..