Description:

Specificity                    Reacts with the heavy chain of mouse IgM

Source                         Pooled antisera from goats hyperimmunized with mouse IgM

Cross Adsorption         Mouse IgG and IgA; may react with immunoglobulins from other species

Purification                  Affinity chromatography on mouse IgM covalently linked to agarose

Applications:

Quality tested applications include –

ELISA 1-6

FLISA

FC 4,7-17

Other referenced applications include –

ELISPOT 6

IHC-FS18、19

IHC-PS 20

ICC  21、22

WB 23,24

Stim 25,26

Working Dilutions:

ELISA        AP conjugate                1:2,000 – 1:4,000

                 HRP conjugate              1:4,000 – 1:8,000

                 BIOT conjugate             1:5,000 – 1:20,000

FLISA        FITC, TRITC, TXRD, AF488, and AF555 conjugates             1:100 – 1:400

                 PE and AF647 conjugates                                                  ≤ 1 μg/mL

Flow Cytometry          FITC, BIOT, and AF488 conjugates                   ≤ 1 μg/10 6 cells

                                   PE and AF647 conjugates                                ≤ 0.1 μg/10 6 cells

                                   For flow cytometry, the suggested use of these reagents is in a final volume of 100 μL

Other Applications     Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

For Research Use Only. Not for Diagnostic or Therapeutic Use.

Handling and Storage：

.The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C.

.The fluorescein (FITC), rhodamine (TRITC), Texas Red ® (TXRD), Alexa Fluor® 488 (AF488), Alexa Fluor® 555 (AF555), and Alexa Fluor® 647 (AF647) conjugates are supplied as 1.0 mg in 1.0 mL of PBS/NaN3. Store at 2-8°C.

.The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL in a stock solution of 50 mM Tris/1 mM MgCl2/50% glycerol, pH 8.0, containing NaN3 as preservative. Store at 2-8°C or long-term at -20°C.

.The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL in a stock solution of 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8°C or long-term at -20°C.

.The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL of PBS/NaN3. Store at 2-8°C.

.The R-phycoerythrin (PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not freeze!

.Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Warning：

Some reagents contain sodium azide. Please refer to product specific SDS.