产品名称

Met (c-Met) Rabbit Polyclonal Antibody

形态

Liquid

WB

IHC-P

WB     1:500

IHC-P  1：200

功能

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Western blot analysis of Met (c-Met) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (KA500242, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (KA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Positive control:

Lane 1: LO2 cell lysate

Lane 2: Hela cell lysate

Lane 3: A549 cell lysate

Lane 4: A431 cell lysate

Lane 5: HT-29 cell lysate