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询盘产品：提取产品名称与信息
产品介绍
产品名称 | Phospho-Met (Y1234/Y1235) Recombinant Rabbit Monoclonal Antibody [PSH06-90] |
抗体类型 | Recombinant Rabbit monoclonal Antibody |
免疫原 | Synthetic phospho-peptide corresponding to residues surrounding Tyr1234/Tyr1235 of human Met aa 1,201-1,250. |
种属反应性 | Human |
验证应用 | WB |
分子量 | Predicted band size: 156 kDa |
阳性对照 | HeLa cell lysate, HeLa starved for 16 hours then treated with 20ng/mL HGF for 10 minutes cell lysate. |
偶联 | unconjugated |
克隆号 | PSH06-90 |
产品特性：
形态 | Liquid |
存放说明 | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
存储缓冲液 | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
亚型 | IgG |
纯化方式 | Protein A affinity purified. |
应用稀释度：
WB | 1:1,000maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm. |
靶点：
 | ☑ Cell treatment (CT) Western blot analysis of Phospho-Met (Y1234/Y1235) on different lysates with Rabbit anti-Phospho-Met (Y1234/Y1235) antibody (KA722765) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa starved for 16 hours then treated with 20ng/mL HGF for 10 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (KA722765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (KA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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