Appearance White powder,Colorless clear liquid after reconstitution
Purity ≥95%,by SDS-PAGE(under reducing(R)&Non-reducing conditions,visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured by its ability to induce Topflash reporter activity in CHO cells in the presence of 50ng/ml wnt3a.The expected EDso is<1.0 μg/ml.
Formulation Lyophilized from a0.22 μm fltered solution containing PBS,5%mannitol and 0.01%Tween 80,pH 7.4
Size 50 μg/vial,1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage &Stability 36 months at -20℃ to -80°℃ in lyophilized state
6 months at -20℃ to-80℃ under sterile conditions after reconstitution
7-10 days at 2℃ to 8℃ under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thawcycles.
Data:
Bioactivity
Measured by its ability to induce Topflash reporter activity in CHO cells in the presence of 50ng/ml wnt3a.The expected EDso is<1.0 μg/ml.The activity of Recombinant Human R-Spondin-1(cat no Y03501)was higher than other competing
products.
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